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Briefly, 50 l of 2-fold serially diluted serum sample was mixed with an equal volume of pseudovirus containing 200 FFU, followed by incubation at 37 C for 2 h

Therefore, serum B 12 values above the cut-off point of deficiency do not necessarily confirm adequate B 12 status

doi: 10.3390/ijms21031047

The original CellTiter-Glo Reagent, once reconstituted, is stable for 48 hours at 4C with approximately 5% signal loss, or 4 days with approximately 20% signal lossrequiring preparation close to the time of use

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